現(xiàn)貨供應(yīng),歡迎訂購: : 胰酶 產(chǎn)品信息: Crystalline Trypsin Solution,貨號 : 03-047-1A/B,胰酶 Cell Dissociation Solution(Non-Enzymatic),貨號: 03-071-1B,(71號) 71號細(xì)胞分離試劑是BI公司*開發(fā)的不含任何酵素、蛋白水解酶及動物組分的一種試劑。運用離子螯合技術(shù)使貼壁細(xì)胞溫和的脫 落,zui大程度地保留完整的細(xì)胞表面蛋白及其生物功能,長時間使用也無需擔(dān)心細(xì)胞受損??蛇m用于血清及不含血清培養(yǎng)細(xì)胞,貼壁更緊密的細(xì)胞可選用木瓜蛋白酶 或結(jié)晶。 Papain Dissociation Solution,貨號 : 03-072-1B, 水晶對細(xì)胞損傷較小、細(xì)胞存活率高,較長時間的使用也無需擔(dān)心。 木瓜蛋白酶與相比有更廣泛的特異性,更廣泛的降解蛋白物質(zhì),效率更高,對細(xì)胞、組織造成的損害更小,極少負(fù)面的影響,同時避免了動物成分的污染。 的使用方法: 1. 使用不含鈣和鎂的PBS溶液沖洗細(xì)胞 2. 每一個T-25培養(yǎng)瓶中加1ml的木瓜蛋白酶分離溶液 3. 15-30°C孵育,當(dāng)細(xì)胞*分離,快速將細(xì)胞懸浮在5-10ml生長培養(yǎng)基中。 4. 離心細(xì)胞,去掉上清 更多相關(guān)產(chǎn)品: BIOLOGICAL INDUSTRIES 05-010-1A "DCCM-1 without L-Glutamine 哺乳動物細(xì)胞懸浮培養(yǎng)無血清培養(yǎng)基" 500ml 4℃ BI公司 05-015-1A "DCCM-2 without L-Glutamine 哺乳動物細(xì)胞懸浮培養(yǎng)無血清培養(yǎng)基" 500ml 4℃ BI公司 05-040-1A "Low Protein Media BSA-Free (LPM), without L-Glutamine 哺乳動物細(xì)胞懸浮培養(yǎng)無血清培養(yǎng)基" 500ml 4℃ BioInd公司 01-015-5A 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